You can fit isotherm equations using the Arbitrary option in the Fit command. We just uploaded this file to the on-line examples.
Here are the settings for the Fit command for a Langmuir isotherm.
For more details on how the non-linear curve fitting works: https://community.visualdatatools.com/datagraph/knowledge-base/command-fit/#arbitrary-fit
We used the form of the equations presented here: https://www2.stetson.edu/~wgrubbs/datadriven/langmuir/langmuirwtg.html
In your case, it sounds like you have time series data. The isotherms are for fitting the values at equilibrium. So it sounds like you might need to use a kinetic model first to determine the equilibrium concentrations. That would also use a non-linear fitting procedure.
Does that help?